This examine presents the primary detection of cardiac troponin I (cTnI), a significant biomarker for acute coronary syndrome analysis (ACS), in human interstitial fluid (ISF) collected through electroporation. Measurements have been carried out utilizing molecularly imprinted polymer nanoparticles as artificial recognition parts and a heat-transfer technique inside a microfluidic system, yielding outcomes inside 15–20 min. This strategy demonstrated dependable cTnI quantification throughout a large, physiologically related focus vary of 0.1 to 1000 pg mL−1 in spiked ISF, reaching a wonderful detection restrict of 1.85 ± 0.32 pg mL−1. Comparisons with standard affected person pattern fluids have been performed by repeating experiments with cTnI-spiked plasma and serum, which exhibited comparable detection limits of 1.78 ± 0.28 and 1.80 ± 0.22 pg mL−1, respectively. The developed sensor affords a fast, extremely delicate, non-invasive, and cost-effective platform for point-of-care ACS analysis in ISF, doubtlessly enhancing affected person outcomes and easing healthcare burdens.
