DNA nanotechnology permits the exact development of intricate nanoscale constructions. Over the previous 20 years, important progress has been made in incorporating dynamic functionalities into these nanostructures. Concurrently, revolutionary methods have emerged for his or her self-assembly and floor patterning into bigger, extra complicated architectures. This evaluate explores the convergence of those two key capabilities-reconfigurability and hierarchical assembly-to engineer DNA origami superstructures with intrinsic dynamic habits. We start by outlining foundational methods in dynamic design, hierarchical meeting, and floor placement, then evaluate latest progress in leveraging these methods to assemble dynamic superstructures with emergent behaviors. The article concludes with a roadmap of main challenges and alternatives shaping the way forward for this quickly evolving area.
