Artificial RNA nanostructures are sometimes composed of 4 nucleotides (A, U, G, and C) following a canonical base pairing rule (A-U/G-C). G·U wobble pairs are generally employed in lots of RNA nanostructures, however different non-canonical base pairing stays underexplored. On this work, we design RNA nanostructures with solely three nucleotides as an alternative of 4. Apart from Watson–Crick G-C base pairs, we incorporate A·C non-canonical base pairs into this three-letter coding scheme and permit selective nanostructure meeting from combined DNA templates. With the brand new paradigm, we produce a wide range of RNA nanostructures, additional increasing the chances of rational molecular design.
