The usage of health-relevant micro organism originating from human microbiomes for the management or remedy of ailments together with neurodegenerative issues or diabetes is at present arising as the subsequent “huge factor” in medication. Directed and fruitful engineering of microbiomes by probiotic supplementation would require a refined, exact and – in all probability much more necessary – simple, quick and handy monitoring of its success e.g. within the sufferers’ intestine. Primarily based on a beforehand described polyclonal SELEX aptamer library advanced towards the human intestine bacterium Blautia producta, we’ve lastly remoted three particular person aptamers, which proofed their efficiency regarding affinity, specificity and robustness in labeling the goal bacterium reliably additionally in mixtures with “contaminating” management micro organism. Used as biofunctionalization molecules on gFETs we may particularly quantify from 101 – 106 cells/mL, may retrace their quantity in mixtures and decided the aptamer Kd-values round 2 nM. These measurements had been doable even within the background of actual human stool pattern. Our outcomes qualify gFETs together with these aptamers BL2, BL7 and BL8 as promising basis for the development of respective sensing units, which then will open new avenues in direction of the meant monitoring approach for probiotic remedy and microbiome engineering approaches.
