This research reveals a extremely promising methodology for developing single-atom nanozymes, significantly well-suited for purposes within the biomedical discipline, similar to glucose detection. Conventional noble steel nanozymes usually current challenges associated to organic toxicity or excessive prices in these purposes. Whereas Fe-based nanozymes exhibit decrease organic toxicity, their preparation usually entails high-temperature circumstances and complicated procedures. On this analysis, a novel strategy was launched through the use of plant extracts as precursors to coordinate single iron atoms, efficiently attaining the synthesis of FeN/GQDs single-atom nanozymes. This methodology not solely simplifies the synthesis course of but additionally considerably reduces its complexity. The ensuing FeN/GQDs single-atom nanozymes not solely reveal excellent peroxidase-like enzyme efficiency but additionally exhibit remarkably excessive sensitivity in glucose detection. This research gives new insights into designing single-atom nanozymes, advancing the synthesis of secure and cost-effective nanozymes. This promising technique holds nice potential for numerous purposes in biosensing and varied biomedical fields.