Gold nanoclusters (AuNCs) are sorts of rising-star fluorescence nanomaterials, however their properties and purposes are hindered by the multi-step synthesis and purification routes in addition to lacks of desired supprting substrates. To be able to strengthen the optical efficiency and dealing efficience, the synthesis and purposes of AuNCs are recommended to be merged collectively on rising substrates. Herein, glutathione-modified hydrophilic rice papers are incubated in chloroauric acid aqueous options and the oxidation-reduction response between glutathione and Au ions allows the in-situ formation of fluorescent AuNCs on the strong fibres of rice papers. The in-situ development of fluoresence AuNCs on rice papers resluted in eye-cataching fluorescence tracks, identical to Chinese language standard calligraphy, and thus the fluoresence calligraphy is outlined on this work. Your complete operation together with synthesis and sign responses is very simple, speedy, and repeatable. Furthermore, the variations of additive chemical reagents in these studied rice papers resulted in responsive fluorescence calligraphy, and people as-synthetic AuNCs supplies exhibited excessive reliabilities and optical stabilities. Considerably, with integration of synchronous formation and purposes of Au nanoclusters on hydrophilic paper substrates, high-performance logical gates and knowledge encryption programs are constructed, remarkably facilitating the progresses of molecular sensing and essential info transmission.